Basic Tissue Processing for Routine Light Microscopy Assignment

Basic Tissue Processing for Routine Light Microscopy - Assignment Example hnique as the tissues are fixed by cross linkages formed by proteins and the cross links does not harm the protein structure and the standard solution is 10% neutral buffered formalin. Glutaraldehyde is also used for fixation and the standard solution is 2% buffered glutaraldehyde. This causes deformations in protein structure and penetrates very slowly but provides overall cytoplasmic and nuclear detail. Alcohols are usually not used for tissues as contain protein denaturants and cause brittleness. Oxidizing agents such as potassium permanganate is also not used frequently as they cross link proteins and cause extensive denaturation. There are number of factors which affect the fixation process such as buffering, penetration, temperature, volume, time interval and concentration of the solutions. Once the tissue has been fixed, they are processed into thin microscopic sections and embedded in paraffin. The technique of fixing tissues into paraffin is called tissue processing. There a re two main steps in the processing which is dehydration and clearing. ‘Dehydration’ is a process of removing water from the tissues as wet fixed tissues cannot be directly infiltrated with paraffin. This is done by mixing alcohol (70%, 95% to 100%) with formalin. Once the tissues are dehydrated, the next step consists of removal of dehydrant with the substance that will be miscible with the paraffin. The common clearing agent used for this step is xylene. The next step involved is that the tissues are finally infiltrated with the embedding agent paraffin. ‘Infiltration’ is a process of impregnating the tissues with the embedding medium. This processed can be automated for large amount of tissues are processed. Automation consists of an instrument that moves through the tissues on a preset time. This activity can also be done with the help of tissue processor by manually picking the tissues out of the cassette and pouring paraffin over them. This ‘emb edding’ process is very

Mitt Romney For President Research Paper Example | Topics and Well Written Essays - 1000 words

Mitt Romney For President - Research Paper Example He married Ann in the year 1969 however the family faced crucial times when she was diagnosed with multiple sclerosis. Although Mitt’s father held the governorship in Michigan however he is not a career politician. Mitt has spent most of his life in the private sector and hence has all the due knowledge of the economy and about its ups and downs. Mitt’s career is a blend of everything ranging from political orientation to economic knowledge, from public serving to turning around things. This is evident from his career how he reversed a state’s declining phase that was caught up in recession. He was also behind the rescue of 2002 Winter Olympic games from the disaster. The exquisite skills that are seen in Mitt’s personality and his management style are attributed to his career in business. After his graduation in 1971 from Brigham University, Mitt got degrees in Law and Business from Harvard. He initially worked as a business consultant but soon in the yea r 1948 he founded the investment firm named Bain Capital. It was this company that helped many firms to rebuild and launch few of them includes: Bright Horizons, Staples and Sports Authority (Mitt Romney for President). The Republican Party i.e. the Grand Old Party (GOP) has its roots back in the United States Democratic Republican Party. The first candidate to be the president under this party had been Abraham Lincoln in 1860 and ever since the party has about 17 Republican presidents who have served about 88 years in total. The party claims to have 55 million registered voters which is the second largest in the country and is also known to be the advocate of American nationalism, conservatism and economics at its core (2012 Republican Candidates). In 2002 when Mitt was elected as the Governor of Massachusetts, the states position was quite alarming as its budget was out of balance, the taxes were increasing, and the spending was also on the rise hence the state’s overall ec onomy was disturbed. Mitt through his supervision and strict decisions reduced the spending and brought it under control. He managed and restructured the government programs and enhanced the efficiencies throughout. This whole turnaround was brought in by Mitt by casting 800 vetoes against the Democrats and by bringing in the conservative principals to state legislature. It was through his hard efforts that the brought job creating incentives removed red tape from businesses and also brought new businesses to the state. He made a huge economic recovery by eliminating the $3 billion deficit without any tax raising or borrowings. At the end of Mitt’s term in 2007 the state has essentially recovered from economic turmoil (Mitt Romney for President). Running for the Republican President Mitt Romney has to confront the other candidates, although his political career is like a weathervane but compared to the other three candidates he is the most experienced choice. His past experie nces as the Governor of Massachusetts, the founder of Bain Capital makes him stand out above competition as his accomplishment and experiences makes him the best choice for the position. In terms of the responsibilities, Mitt has that skill in him to get things done as seen in the 2002 Salt Lake City Olympics games. Since he has spent most of his career in the private sector, Mitt has a lot to offer as he understands the economy in its very true sense. He has built up his career by starting new